Paul Guichard

Publications

2019

Screen Shot 2019-11-11 at 20.01.43.png31. A helical inner scaffold provides a structural basis for centriole cohesion. 
Le Guennec M, Klena N., Gambarotto D., Laporte MH , Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.Science Advances (accepted)

 

Screen Shot 2019-09-28 at 17.34.28.png 30. The RB530 antibody recognizes microtubules by immunofluorescence
Eloïse Bertiaux-Lequoy, Philippe Hammel, Virginie Hamel, Paul Guichard. Antibody reports. Sept 2019

Link open access

 

Screen Shot 2019-05-03 at 17.47.01.png29. Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*. Science. Jan 2019
Link open access ReprintFull text 
 

Screen Shot 2019-05-03 at 16.58.37.png

28. Imaging cellular ultrastructures using expansion microscopy (U-ExM).  Gambarotto D. ##, Zwettler F. U. ##, Schmidt-Cernohorska M. , Fortun D. , Borgers  S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and  Guichard P.*. Nature Methods. 2019
Link open access and Preprint

 

2018

Screen Shot 2019-05-03 at 22.58.26.png27. Isolation, Imaging and fluorescent single particle reconstruction of Chlamydomonas centrioles. Klena1 N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.* JoVE. (139), e58109, doi:10.3791/58109

Open Access

 

Screen Shot 2019-05-03 at 17.10.22.png26. Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun DGuichard PHamel VSorzano COSBanterle NGonczy PUnser MIEEE Trans Med Imaging. 2018 May;37(5):1235-1246. 

Open Access

 

Screen Shot 2019-05-03 at 17.15.24.png25. Super-resolution microscopy to decipher multi-molecular assemblies. Sieben CDouglass KMGuichard PManley S. Curr Opin Struct Biol. 2018 Apr;49:169-176. 

 

 

Screen Shot 2019-05-03 at 17.18.01.png24. The Rise of the Cartwheel: Seeding the Centriole Organelle. Guichard PHamel VGönczy PBioessays. 2018 Apr;40(4):e1700241. 

Open Access

 

 2017

Screen Shot 2019-05-03 at 17.19.47.png23. TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity. Prouteau MDesfosses ASieben CBourgoint CLydia Mozaffari NDemurtas DMitra AKGuichard PManley SLoewith R. Nature. 2017 Oct 12;550(7675):265-269. 

 

Screen Shot 2019-05-03 at 17.20.09.png22. Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel VSteib EHamelin RArmand FBorgers SFlückiger IBusso COlieric NSorzano COSSteinmetz MOGuichard P*Gönczy P.*  Curr Biol. 2017 Aug 21;27(16):2486-2498.e6.

 Open Access

 

Screen Shot 2019-05-03 at 17.28.08.png21. Cell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard PHamel VLe Guennec MBanterle NIacovache INemčíková VFlückiger IGoldie KNStahlberg HLévy DZuber BGönczy PNat Commun. 2017 Mar 23;8:14813.  

Open Access

 

2016 

Screen Shot 2019-05-03 at 17.31.00.png20. The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding. Kraatz SGuichard PObbineni JMOlieric NHatzopoulos GNHilbert MSen IMissimer JGönczy PSteinmetz MOStructure. 2016 Aug 2;24(8):1358-1371. 

 Open Access

 

Screen Shot 2019-05-03 at 17.32.56.png19. Computational support for a scaffolding mechanism of centriole assembly. Klein HCGuichard PHamel VGönczy PSchwarz USSci Rep. 2016 Jun 8;6:27075.  

Open Access

 

D Fortun, P Guichard, N Chu, M Unser - IEEE Journal of Selected Topics in Signal Processing, 2016
 
 
 

Screen Shot 2019-05-03 at 17.35.46.png17. SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert MNoga AFrey DHamel VGuichard PKraatz SHPfreundschuh MHosner SFlückiger IJaussi RWieser MMThieltges KMDeupi XMüller DJKammerer RAGönczy PHirono MSteinmetz MONat Cell Biol. 2016 Apr;18(4):393-403. 

 

Screen Shot 2019-05-03 at 17.36.27.png16. Basal body structure in Trichonympha. Guichard PGönczy PCilia. 2016 Mar 1;5:9. Review. 

Open Access

 

 

2015 

Screen Shot 2019-05-03 at 17.40.31.png15. Direct visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography. Demurtas DGuichard PMartiel IMezzenga RHébert CSagalowicz LNat Commun. 2015 Nov 17;6:8915. 

Open Access

 

Screen Shot 2019-05-03 at 18.24.00.png14. Isolation, cryotomography, and three-dimensional reconstruction of centrioles. Guichard PHamel VNeves AGönczy PMethods Cell Biol. 2015;129:191-209. 

 

 

Screen Shot 2019-05-03 at 18.20.28.png13. Purification of centrosomes from mammalian cell lines. Gogendeau DGuichard PTassin AMMethods Cell Biol. 2015;129:171-89. 

 

 
 2014

Screen Shot 2019-05-03 at 17.43.03.png12. Correlative multicolor 3D SIM and STORM microscopy. Hamel VGuichard PFournier MGuiet RFlückiger ISeitz AGönczy PBiomed Opt Express. 2014 Aug 29;5(10):3326-36. 

Open Access 

 

 2013

Screen Shot 2019-05-03 at 17.43.19.png11. Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard PHachet VMajubu NNeves ADemurtas DOlieric NFluckiger IYamada AKihara KNishida YMoriya SSteinmetz MOHongoh YGönczy PCurr Biol. 2013 Sep 9;23(17):1620-8. 

 

Screen Shot 2019-05-03 at 18.36.35.png10. Hepatitis B subvirus particles display both a fluid bilayer membrane and a strong resistance to freeze drying: a study by solid-state NMR, light scattering, and cryo-electron microscopy/tomography. Grélard AGuichard PBonnafous PMarco SLambert OManin CRonzon FDufourc EJFASEB J. 2013 Oct;27(10):4316-26. 

 

Screen Shot 2019-05-03 at 18.37.47.png9. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert MErat MCHachet VGuichard PBlank IDFlückiger ISlater LLowe EDHatzopoulos GNSteinmetz MOGönczy PVakonakis IProc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. 

 

Screen Shot 2019-05-03 at 18.40.34.png8. An atomistic view of microtubule stabilization by GTP. Quiniou EGuichard PPerahia DMarco SMouawad LStructure. 2013 May 7;21(5):833-43. 

 

 

Screen Shot 2019-05-03 at 21.39.18.png7. Use of red autofluorescence for monitoring prodiginine biosynthesis. Tenconi EGuichard PMotte PMatagne ARigali SJ Microbiol Methods. 2013 May;93(2):138-43. doi: 10.1016/j.mimet.2013.02.012. Epub 2013 Mar 18.

 

 
2012

Screen Shot 2019-05-03 at 18.49.33.png6. Cartwheel architecture of Trichonympha basal body. Guichard PDesfosses AMaheshwari AHachet VDietrich CBrune AIshikawa TSachse CGönczy PScience. 2012 Aug 3;337(6094):553. 

 

 

2011

Screen Shot 2019-05-03 at 22.06.40.png5. Three dimensional morphology of rabies virus studied by cryo-electron tomography. Guichard PKrell TChevalier MVaysse CAdam ORonzon FMarco SJ Struct Biol. 2011 Oct;176(1):32-40. 

 

 
2010

Screen Shot 2019-05-03 at 22.10.54.png4. Procentriole assembly revealed by cryo-electron tomography. Guichard PChrétien DMarco STassin AMEMBO J. 2010 May 5;29(9):1565-72. 

 

 

Screen Shot 2019-05-03 at 22.18.37.png3. Self-assembling SAS-6 multimer is a core centriole building block. Gopalakrishnan JGuichard PSmith AHSchwarz HAgard DAMarco SAvidor-Reiss T. J Biol Chem. 2010 Mar 19;285(12):8759-70. 

 
 
 
 2009

Screen Shot 2019-05-03 at 22.22.08.png2. Involvement of HFq protein in the post-transcriptional regulation of E. coli bacterial cytoskeleton and cell division proteins. Zambrano NGuichard PPBi YCayrol BMarco SArluison VCell Cycle. 2009 Aug;8(15):2470-2. 

 

 

Screen Shot 2019-05-03 at 22.23.33.png1. Visualization of proteins in intact cells with a clonable tag for electron microscopy. Diestra EFontana JGuichard PMarco SRisco CJ Struct Biol. 2009 Mar;165(3):157-68.