Paul Guichard

POBdb

POBdb (Proteome of Basal body database): http://pobdb.vital-it.ch/

We performed a proteomic analysis of isolated Chlamydomonas basal bodies (centrioles) to identify new candidate basal body proteins using a mild isolation protocol that allows notable preservation of the procentriole and of its cartwheel. We used a dimethyl labeling approach that enables one to conduct relative quantitative analysis of protein mixtures present in two samples; in this manner, we aimed at subtracting potential contaminants and thus identifying bona fide centriolar proteins.

This web resource has been developed to allow full access to the mass spectrometry data set from this project to the scientific community. Users can navigate through the graphical view of the results ("data" section) and obtain further information on the identified proteins and their homologues in other species, as well as BLAST a protein of interest against the proteins identified in this work.