Guichard / Hamel



Screen Shot 2020-07-09 at 21.26.56.pngExpansion Microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid structure. Bertiaux A., Balestra A., Bournonville L., Brochet M. *, Guichard P. *, Hamel V. * 

PLOS Biology. Link open accessBiorXiv - Prelights


Screen Shot 2020-07-05 at 17.40.49.png Characterization of the Novel Mitochondrial Genome Segregation Factor TAP110 in Trypanosoma brucei.

Amodeo S.Kalichava A.Fradera-Sola A.Bertiaux-Lequoy E.Guichard P.Butter F.Ochsenreiter T.
Journal of Cell Science. Link JCS BiorXiv - Prelights
Le Guennec M.†, Klena N.†, Hamel V.* and Guichard P.*
Current Opinion in Structural Biology. Review. February 2021

Screen Shot 2020-04-29 at 20.34.03.pngThe architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

Klena N., Le Guennec M., Tassin AM, Van den Hoek H..Erdmann PS, Schaffer M., Geimer S., Aeschlimann G., Kovacik L., Goldie KN,  Stahlberg H. , Engel BD*, Hamel V.* and Guichard P.
EMBO J. September 2020 - Link open access- Link BiorXiv

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Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Nazarov S.Bezler A.Hatzopoulos G.,Nemčíková Villímová V.Demurtas D.Le Guennec M.Guichard P.Gönczy P.  

EMBO J. September 2020 - Link Open Access - Link BiorXiv.


 Steib E., Laporte MH, Gambarotto D., Olieric N., Zheng C., Borgers S., Olieric V., Le Guennec M., Koll F., Tassin AM, Steinmetz MO, Guichard P.*, Hamel V.*  

eLIFE, September 2020. Link open access - link BioRxiv


Screen Shot 2020-07-05 at 17.55.14.pngImproving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy.

Hamel V.* and Guichard P.*

Methods in Cell Biology, August 2020. Link


Gambarotto D., Hamel V.*Guichard P.*
Methods in Cell Biology, July 2020. Link
Screen Shot 2020-03-13 at 18.30.16.jpgMolecular resolution imaging by post-labeling expansion single molecular localization microscopy (Ex-SMLM).Zwettler F.U.Reinhard S.Gambarotto D., Bell T.D.MHamel V.*Guichard P.*Sauer M.* 
Nature communications., July 2020. Link open access - BiorXivPrelights - Press release
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Homogeneous multifocal excitation for high-throughput super-resolution imaging.Mahecic D., Gambarotto D., Douglass K.M., Fortun D., Banterle N., Le Guennec M., Ibrahim K., Gonczy P., Hamel V., Guichard P.., Manley S.
Nature Methods, June 2020. Link open access
 Tosetti N., Dos Santos Pacheco N., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. 
eLife. May 2020 Link open access
Screen Shot 2019-11-11 at 20.01.43.pngA helical inner scaffold provides a structural basis for centriole cohesion. Le Guennec M, Klena N., Gambarotto D., Laporte MH , Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.
Science Advances. Feb 2020 Link open access - Unige press release



Screen Shot 2019-09-28 at 17.34.28.png The RB530 antibody recognizes microtubules by immunofluorescence
Eloïse Bertiaux-Lequoy, Philippe Hammel, Virginie Hamel, Paul Guichard.
Antibody reports. Sept 2019. Link open access



Screen Shot 2019-05-03 at 17.47.01.pngFlagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*.
Science. Jan 2019. Link open access ReprintFull text 


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Imaging cellular ultrastructures using expansion microscopy (U-ExM).  Gambarotto D. ##, Zwettler F. U. ##, Schmidt-Cernohorska M. , Fortun D. , Borgers  S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and  Guichard P.*. 
Nature Methods. 2019. Link open access - Preprint - Prelights - Press release




Screen Shot 2019-05-03 at 22.58.26.pngIsolation, Imaging and fluorescent single particle reconstruction of Chlamydomonas centrioles. Klena1 N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.* 

JoVE. (139), e58109, doi:10.3791/58109. Open Access


Screen Shot 2019-05-03 at 17.10.22.pngReconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun DGuichard PHamel VSorzano COSBanterle NGonczy PUnser M

IEEE Trans Med Imaging. 2018 May;37(5):1235-1246.  Open Access


Screen Shot 2019-05-03 at 17.15.24.pngSuper-resolution microscopy to decipher multi-molecular assemblies. Sieben CDouglass KMGuichard PManley S. 
Curr Opin Struct Biol. 2018 Apr;49:169-176. 



Bioessays. 2018 Apr;40(4):e1700241.  Open Access



Screen Shot 2019-05-03 at 17.19.47.pngTORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity. Prouteau MDesfosses ASieben CBourgoint CLydia Mozaffari NDemurtas DMitra AKGuichard PManley SLoewith R. 
Nature. 2017 Oct 12;550(7675):265-269. Unige press release



Screen Shot 2019-05-03 at 17.20.09.pngIdentification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel VSteib EHamelin RArmand FBorgers SFlückiger IBusso COlieric NSorzano COSSteinmetz MOGuichard P*Gönczy P.*  
Curr Biol. 2017 Aug 21;27(16):2486-2498.e6. Open Access


Screen Shot 2019-05-03 at 17.28.08.pngCell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard PHamel VLe Guennec MBanterle NIacovache INemčíková VFlückiger IGoldie KNStahlberg HLévy DZuber BGönczy P
Nat Commun. 2017 Mar 23;8:14813.  Open Access



Screen Shot 2019-05-03 at 17.31.00.pngThe Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding. Kraatz SGuichard PObbineni JMOlieric NHatzopoulos GNHilbert MSen IMissimer JGönczy PSteinmetz MO
Structure. 2016 Aug 2;24(8):1358-1371. Open Access


Klein HCGuichard PHamel VGönczy PSchwarz US
Sci Rep. 2016 Jun 8;6:27075.  Open Access
D Fortun, P Guichard, N Chu, M Unser 
IEEE Journal of Selected Topics in Signal Processing, 2016
Screen Shot 2019-05-03 at 17.35.46.pngSAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert MNoga AFrey DHamel VGuichard PKraatz SHPfreundschuh MHosner SFlückiger IJaussi RWieser MMThieltges KMDeupi XMüller DJKammerer RAGönczy PHirono MSteinmetz MO
Nat Cell Biol. 2016 Apr;18(4):393-403. 


Guichard PGönczy P
Cilia. 2016 Mar 1;5:9. Review. Open Access




Screen Shot 2019-05-03 at 17.40.31.pngDirect visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography. Demurtas DGuichard PMartiel IMezzenga RHébert CSagalowicz L
Nat Commun. 2015 Nov 17;6:8915. Open Access



Guichard PHamel VNeves AGönczy P
Methods Cell Biol. 2015;129:191-209. 



Gogendeau DGuichard PTassin AM
Methods Cell Biol. 2015;129:171-89. 


Hamel VGuichard PFournier MGuiet RFlückiger ISeitz AGönczy P
Biomed Opt Express. 2014 Aug 29;5(10):3326-36. Open Access 



Screen Shot 2019-05-03 at 17.43.19.pngNative architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard PHachet VMajubu NNeves ADemurtas DOlieric NFluckiger IYamada AKihara KNishida YMoriya SSteinmetz MOHongoh YGönczy P
Curr Biol. 2013 Sep 9;23(17):1620-8. 


Screen Shot 2019-05-03 at 18.36.35.pngHepatitis B subvirus particles display both a fluid bilayer membrane and a strong resistance to freeze drying: a study by solid-state NMR, light scattering, and cryo-electron microscopy/tomography. Grélard AGuichard PBonnafous PMarco SLambert OManin CRonzon FDufourc EJ

FASEB J. 2013 Oct;27(10):4316-26. 


Screen Shot 2019-05-03 at 18.37.47.pngCaenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert MErat MCHachet VGuichard PBlank IDFlückiger ISlater LLowe EDHatzopoulos GNSteinmetz MOGönczy PVakonakis IProc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. 


Quiniou EGuichard PPerahia DMarco SMouawad L.
Structure. 2013 May 7;21(5):833-43. 



Screen Shot 2019-05-03 at 21.39.18.pngUse of red autofluorescence for monitoring prodiginine biosynthesis. Tenconi EGuichard PMotte PMatagne ARigali SJ Microbiol Methods. 2013 May;93(2):138-43. doi: 10.1016/j.mimet.2013.02.012. Epub 2013 Mar 18.
Screen Shot 2019-05-03 at 18.49.33.pngCartwheel architecture of Trichonympha basal body. Guichard PDesfosses AMaheshwari AHachet VDietrich CBrune AIshikawa TSachse CGönczy P
Science. 2012 Aug 3;337(6094):553. 



Screen Shot 2019-05-03 at 22.06.40.pngThree dimensional morphology of rabies virus studied by cryo-electron tomography. Guichard PKrell TChevalier MVaysse CAdam ORonzon FMarco SJ Struct Biol. 2011 Oct;176(1):32-40. 


Guichard PChrétien DMarco STassin AM
EMBO J. 2010 May 5;29(9):1565-72. 



Screen Shot 2019-05-03 at 22.18.37.pngSelf-assembling SAS-6 multimer is a core centriole building block. Gopalakrishnan JGuichard PSmith AHSchwarz HAgard DAMarco SAvidor-Reiss T. 
J Biol Chem. 2010 Mar 19;285(12):8759-70. 

Screen Shot 2019-05-03 at 22.22.08.pngInvolvement of HFq protein in the post-transcriptional regulation of E. coli bacterial cytoskeleton and cell division proteins. Zambrano NGuichard PPBi YCayrol BMarco SArluison VCell Cycle. 2009 Aug;8(15):2470-2. 



Screen Shot 2019-05-03 at 22.23.33.pngVisualization of proteins in intact cells with a clonable tag for electron microscopy. Diestra EFontana JGuichard PMarco SRisco CJ Struct Biol. 2009 Mar;165(3):157-68.