Guichard / Hamel

Publications

2020

Screen Shot 2020-07-19 at 13.21.50.png

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Nazarov S.Bezler A.Hatzopoulos G.,Nemčíková Villímová V.Demurtas D.Le Guennec M.Guichard P.Gönczy P.  

BiorXiv.

 

Screen Shot 2020-07-09 at 21.26.56.pngExpansion Microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid structure. Bertiaux A., Balestra A., Bournonville L., Brochet M. *, Guichard P. *, Hamel V. * 

BiorXiv - Prelights

  

Screen Shot 2020-07-05 at 17.40.49.png Characterization of the Novel Mitochondrial Genome Segregation Factor TAP110 in Trypanosoma brucei.

Amodeo S.Kalichava A.Fradera-Sola A.Bertiaux-Lequoy E.Guichard P.Butter F.Ochsenreiter T.
 
 

Screen Shot 2020-04-29 at 20.34.03.pngThe architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

Klena N., Le Guennec M., Tassin AM, Van den Hoek H..Erdmann PS, Schaffer M., Geimer S., Aeschlimann G., Kovacik L., Goldie KN,  Stahlberg H. , Engel BD*, Hamel V.* and Guichard P.
 
 
Screen Shot 2020-02-15 at 13.07.07.pngWDR90 is a centriolar microtubule wall protein important for centriole architecture integrity

 Steib E., Gambarotto D., Laporte MH, Olieric N., Zheng C., Borgers S., Olieric V., Le Guennec M., Koll F., Tassin AM, Steinmetz MO, Guichard P.*, Hamel V.*  BioRxiv

 

Screen Shot 2020-07-05 at 17.55.14.pngImproving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy.

Hamel V.* and Guichard P.*

Methods in Cell Biology, In press

 

Gambarotto D., Hamel V.*Guichard P.*
Methods in Cell Biology, July 2020. Link
 
 
 
Screen Shot 2020-03-13 at 18.30.16.jpgMolecular resolution imaging by post-labeling expansion single molecular localization microscopy (Ex-SMLM).Zwettler F.U.Reinhard S.Gambarotto D., Bell T.D.MHamel V.*Guichard P.*Sauer M.* Nature communications., July 2020. Link open access - BiorXivPrelights - Press release
 
 
Screen Shot 2020-05-13 at 18.17.47.jpg
Homogeneous multifocal excitation for high-throughput super-resolution imaging.Mahecic D., Gambarotto D., Douglass K.M., Fortun D., Banterle N., Le Guennec M., Ibrahim K., Gonczy P., Hamel V., Guichard P.., Manley S. Nature Methods, June 2020. Link open access
 
 
 Tosetti N., Dos Santos Pacheco N., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. eLife. May 2020 Link open access
 
 

Screen Shot 2019-11-11 at 20.01.43.pngA helical inner scaffold provides a structural basis for centriole cohesion. 

Le Guennec M, Klena N., Gambarotto D., Laporte MH , Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.Science Advances. Feb 2020 Link open access - Unige press release

 

2019

Screen Shot 2019-09-28 at 17.34.28.png The RB530 antibody recognizes microtubules by immunofluorescence

Eloïse Bertiaux-Lequoy, Philippe Hammel, Virginie Hamel, Paul Guichard. Antibody reports. Sept 2019

Link open access

 

Screen Shot 2019-05-03 at 17.47.01.pngFlagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*. Science. Jan 2019

Link open access ReprintFull text 

 

Screen Shot 2019-05-03 at 16.58.37.png

Imaging cellular ultrastructures using expansion microscopy (U-ExM).  Gambarotto D. ##, Zwettler F. U. ##, Schmidt-Cernohorska M. , Fortun D. , Borgers  S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and  Guichard P.*. Nature Methods. 2019

Link open access - Preprint - Prelights - Press release

 

 

2018

Screen Shot 2019-05-03 at 22.58.26.pngIsolation, Imaging and fluorescent single particle reconstruction of Chlamydomonas centrioles. Klena1 N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.* JoVE. (139), e58109, doi:10.3791/58109

Open Access

 

Screen Shot 2019-05-03 at 17.10.22.pngReconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun DGuichard PHamel VSorzano COSBanterle NGonczy PUnser MIEEE Trans Med Imaging. 2018 May;37(5):1235-1246. 

Open Access

 

Screen Shot 2019-05-03 at 17.15.24.pngSuper-resolution microscopy to decipher multi-molecular assemblies. Sieben CDouglass KMGuichard PManley S. Curr Opin Struct Biol. 2018 Apr;49:169-176. 

 

 

Screen Shot 2019-05-03 at 17.18.01.pngThe Rise of the Cartwheel: Seeding the Centriole Organelle. Guichard PHamel VGönczy PBioessays. 2018 Apr;40(4):e1700241. 

Open Access

 

 2017

Screen Shot 2019-05-03 at 17.19.47.pngTORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity. Prouteau MDesfosses ASieben CBourgoint CLydia Mozaffari NDemurtas DMitra AKGuichard PManley SLoewith R. Nature. 2017 Oct 12;550(7675):265-269. 

Unige press release

 

 

Screen Shot 2019-05-03 at 17.20.09.pngIdentification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel VSteib EHamelin RArmand FBorgers SFlückiger IBusso COlieric NSorzano COSSteinmetz MOGuichard P*Gönczy P.*  Curr Biol. 2017 Aug 21;27(16):2486-2498.e6.

 Open Access

 

Screen Shot 2019-05-03 at 17.28.08.pngCell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard PHamel VLe Guennec MBanterle NIacovache INemčíková VFlückiger IGoldie KNStahlberg HLévy DZuber BGönczy PNat Commun. 2017 Mar 23;8:14813.  

Open Access

 

2016 

Screen Shot 2019-05-03 at 17.31.00.pngThe Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding. Kraatz SGuichard PObbineni JMOlieric NHatzopoulos GNHilbert MSen IMissimer JGönczy PSteinmetz MOStructure. 2016 Aug 2;24(8):1358-1371. 

 Open Access

 

Screen Shot 2019-05-03 at 17.32.56.pngComputational support for a scaffolding mechanism of centriole assembly. Klein HCGuichard PHamel VGönczy PSchwarz USSci Rep. 2016 Jun 8;6:27075.  

Open Access

 

D Fortun, P Guichard, N Chu, M Unser - IEEE Journal of Selected Topics in Signal Processing, 2016
 
 
 

Screen Shot 2019-05-03 at 17.35.46.pngSAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert MNoga AFrey DHamel VGuichard PKraatz SHPfreundschuh MHosner SFlückiger IJaussi RWieser MMThieltges KMDeupi XMüller DJKammerer RAGönczy PHirono MSteinmetz MONat Cell Biol. 2016 Apr;18(4):393-403. 

 

Screen Shot 2019-05-03 at 17.36.27.pngBasal body structure in Trichonympha. Guichard PGönczy PCilia. 2016 Mar 1;5:9. Review. 

Open Access

 

 

2015 

Screen Shot 2019-05-03 at 17.40.31.pngDirect visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography. Demurtas DGuichard PMartiel IMezzenga RHébert CSagalowicz LNat Commun. 2015 Nov 17;6:8915. 

Open Access

 

Screen Shot 2019-05-03 at 18.24.00.pngIsolation, cryotomography, and three-dimensional reconstruction of centrioles. Guichard PHamel VNeves AGönczy PMethods Cell Biol. 2015;129:191-209. 

 

 

Screen Shot 2019-05-03 at 18.20.28.pngPurification of centrosomes from mammalian cell lines. Gogendeau DGuichard PTassin AMMethods Cell Biol. 2015;129:171-89. 

 

 
 2014

Screen Shot 2019-05-03 at 17.43.03.pngCorrelative multicolor 3D SIM and STORM microscopy. Hamel VGuichard PFournier MGuiet RFlückiger ISeitz AGönczy PBiomed Opt Express. 2014 Aug 29;5(10):3326-36. 

Open Access 

 

 2013

Screen Shot 2019-05-03 at 17.43.19.pngNative architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard PHachet VMajubu NNeves ADemurtas DOlieric NFluckiger IYamada AKihara KNishida YMoriya SSteinmetz MOHongoh YGönczy PCurr Biol. 2013 Sep 9;23(17):1620-8. 

 

Screen Shot 2019-05-03 at 18.36.35.pngHepatitis B subvirus particles display both a fluid bilayer membrane and a strong resistance to freeze drying: a study by solid-state NMR, light scattering, and cryo-electron microscopy/tomography. Grélard AGuichard PBonnafous PMarco SLambert OManin CRonzon FDufourc EJFASEB J. 2013 Oct;27(10):4316-26. 

 

Screen Shot 2019-05-03 at 18.37.47.pngCaenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert MErat MCHachet VGuichard PBlank IDFlückiger ISlater LLowe EDHatzopoulos GNSteinmetz MOGönczy PVakonakis IProc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. 

 

Screen Shot 2019-05-03 at 18.40.34.pngAn atomistic view of microtubule stabilization by GTP. Quiniou EGuichard PPerahia DMarco SMouawad LStructure. 2013 May 7;21(5):833-43. 

 

 

Screen Shot 2019-05-03 at 21.39.18.pngUse of red autofluorescence for monitoring prodiginine biosynthesis. Tenconi EGuichard PMotte PMatagne ARigali SJ Microbiol Methods. 2013 May;93(2):138-43. doi: 10.1016/j.mimet.2013.02.012. Epub 2013 Mar 18.

 

 
2012

Screen Shot 2019-05-03 at 18.49.33.pngCartwheel architecture of Trichonympha basal body. Guichard PDesfosses AMaheshwari AHachet VDietrich CBrune AIshikawa TSachse CGönczy PScience. 2012 Aug 3;337(6094):553. 

 

 

2011

Screen Shot 2019-05-03 at 22.06.40.pngThree dimensional morphology of rabies virus studied by cryo-electron tomography. Guichard PKrell TChevalier MVaysse CAdam ORonzon FMarco SJ Struct Biol. 2011 Oct;176(1):32-40. 

 

 
2010

Screen Shot 2019-05-03 at 22.10.54.pngProcentriole assembly revealed by cryo-electron tomography. Guichard PChrétien DMarco STassin AMEMBO J. 2010 May 5;29(9):1565-72. 

 

 

 

Screen Shot 2019-05-03 at 22.18.37.pngSelf-assembling SAS-6 multimer is a core centriole building block. Gopalakrishnan JGuichard PSmith AHSchwarz HAgard DAMarco SAvidor-Reiss T. J Biol Chem. 2010 Mar 19;285(12):8759-70. 

 
 
 
 2009

Screen Shot 2019-05-03 at 22.22.08.pngInvolvement of HFq protein in the post-transcriptional regulation of E. coli bacterial cytoskeleton and cell division proteins. Zambrano NGuichard PPBi YCayrol BMarco SArluison VCell Cycle. 2009 Aug;8(15):2470-2. 

 

 

Screen Shot 2019-05-03 at 22.23.33.pngVisualization of proteins in intact cells with a clonable tag for electron microscopy. Diestra EFontana JGuichard PMarco SRisco CJ Struct Biol. 2009 Mar;165(3):157-68.