Guichard / Hamel

Virginie Hamel

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Dr. Virginie Hamel

Co-group leader - Collaboratrice Scientifique

2017
+41 22 379 6735
E-mail


MINI CV

Since 2015 - Co-group leader, UNIGE

2012-2015 - Staff Scientist at EPFL (Pierre Gonczy's lab)

2005- 2012 - Post-doc at EPFL (Pierre Gonczy's lab)

2004 - PhD at EMBL (Iain Mattaj's lab)

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FELLOWSHIPS AND GRANTS

2008-2010 -  Marie Heim Vögtlin fellowshio (SNSF fellowship)

2008-2009 - Roche foundation fellowship

2005-2008 - FEBS longterm fellowship

2003-2004 - EMBL Fellowship

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Publications 

28. Expansion Microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid structure. Bertiaux A., Balestra A., Bournonville L., Brochet M. *, Guichard P. *, Hamel V. * . Submitted

 

27. The architecture of the centriole cartwheel-containing region revealed by cryo-electron tomographyKlena N.†, Le Guennec M.†, Tassin AM, Van den Hoek H., .Erdmann PS, Schaffer M., Geimer S., Aeschlimann G., Kovacik L., Goldie KN,  Stahlberg H. , Engel BD*, Hamel V.* and Guichard P.*  Submitted.  

 

26. WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. Steib E., Gambarotto D., Laporte MH, Olieric N., Zheng C., Borgers S., Olieric V., Le Guennec M., Koll F., Tassin AM, Steinmetz MO, Guichard P.*, Hamel V.*  Submitted. BioRxiv

 

25. Ultrastructure Expansion Microscopy (U-ExM).Gambarotto D., Hamel V.*Guichard P.*Methods in Cell Biology. July 2020. 

 

24. Improving the resolution of fluorescence nanoscopy using post-expansion labeling microscopyHamel V.* and Guichard P.* Methods in Cell Biology, In press

 

23. Molecular resolution imaging by post-labeling expansion single molecular localization microscopy (Ex-SMLM).Zwettler F.U.Reinhard S.Gambarotto D., Bell T.D.MHamel V.*Guichard P.*Sauer M.* Nature communications. July 2020. 

 

22. Homogeneous multifocal excitation for high-throughput super-resolution imaging.Mahecic D., Gambarotto D., Douglass K.M., Fortun D., Banterle N., Le Guennec M., Ibrahim K., Gonczy P., Hamel V., Guichard P.., Manley S. Nature Methods, June 2020. Link open access

 

 Tosetti N., Dos Santos Pacheco N., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. eLifeMay 2020 Link open access

 

20. A helical inner scaffold provides a structural basis for centriole cohesion. Le Guennec M, Klena N., Gambarotto D., Laporte MH , Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.Science Advances. Feb 2020

 

19. Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*. Science. Jan 2019

 

18. Imaging cellular ultrastructures using expansion microscopy (U-ExM).  Gambarotto D. ##, Zwettler F. U. ##, Le Guennec M., Schmidt-Cernohorska M. , Fortun D. , Borgers  S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and  Guichard P.*. Nature Methods. 2019

 

17. Isolation, Imaging and fluorescent single particle reconstruction of  Chlamydomonas  centrioles . Klena N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.JoVE(139), e58109, doi:10.3791/58109

 

16. Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun DGuichard PHamel VSorzano COSBanterle NGonczy PUnser MIEEE Trans Med Imaging. 2018 May;37(5):1235-1246. 

 

15.  Rise of the Cartwheel: Seeding the Centriole Organelle. Guichard PHamel VGönczy PBioessays. 2018 Apr;40(4):e1700241. 

 

14. Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel V, Steib EHamelin RArmand FBorgers SFlückiger IBusso COlieric NSorzano COSSteinmetz MOGuichard P*Gönczy P.* Curr Biol. 2017 Aug 21;27(16):2486-2498.e6. 

 

13. Cell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard P*Hamel V*Le Guennec MBanterle NIacovache INemčíková VFlückiger IGoldie KNStahlberg HLévy DZuber BGönczy PNat Commun. 2017 Mar 23;8:14813.

 

12. Computational support for a scaffolding mechanism of centriole assembly. Klein HCGuichard PHamel V, Gönczy PSchwarz USSci Rep. 2016 Jun 8;6:27075. 

 

11. SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert M*Noga A*Frey D*Hamel V*Guichard PKraatz SHPfreundschuh MHosner SFlückiger IJaussi RWieser MMThieltges KMDeupi XMüller DJKammerer RAGönczy PHirono MSteinmetz MONat Cell Biol. 2016 Apr;18(4):393-403. 

 

10. Isolation, cryotomography, and three-dimensional reconstruction of centrioles. Guichard PHamel V, Neves AGönczy PMethods Cell Biol. 2015;129:191-209. 

 

9. Correlative multicolor 3D SIM and STORM microscopy. Hamel VGuichard PFournier MGuiet RFlückiger ISeitz AGönczy PBiomed Opt Express. 2014 Aug 29;5(10):3326-36. 

 

8. Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. Keller DOrpinell MOlivier NWachsmuth MMahen RWyss RHachet VEllenberg JManley SGönczy P. J Cell Biol. 2014 Mar 3;204(5):697-712. 

 

7. Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard PHachet VMajubu NNeves ADemurtas DOlieric NFluckiger IYamada AKihara KNishida YMoriya SSteinmetz MOHongoh YGönczy PCurr Biol. 2013 Sep 9;23(17):1620-8. 

 

6. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert M*Erat MC*Hachet V*Guichard PBlank IDFlückiger ISlater LLowe EDHatzopoulos GNSteinmetz MOGönczy PVakonakis IProc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. 

 

5. Cartwheel architecture of Trichonympha basal body. Guichard PDesfosses AMaheshwari AHachet V, Dietrich CBrune AIshikawa TSachse CGönczy PScience. 2012 Aug 3;337(6094):553. 

 

4. The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos. Hachet VBusso CToya MSugimoto AAskjaer PGönczy PMol Biol Cell. 2012 Aug;23(16):3111-21. 

 

3. Centrosomes promote timely mitotic entry in C. elegans embryos. Hachet VCanard CGönczy PDev Cell. 2007 Apr;12(4):531-41.

 

2. Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3. Faul CHüttelmaier SOh J, Hachet VSinger RHMundel P. J Cell Biol. 2005 May 9;169(3):415-24.

 

1. Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro. Hachet VKöcher TWilm MMattaj IWEMBO J. 2004 Apr 7;23(7):1526-35.