Virginie Hamel
MINI CV
Since 2015 - Co-group leader, UNIGE
2012-2015 - Staff Scientist at EPFL (Pierre Gonczy's lab)
2005- 2012 - Post-doc at EPFL (Pierre Gonczy's lab)
2004 - PhD at EMBL (Iain Mattaj's lab)
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FELLOWSHIPS AND GRANTS
2008-2010 - Marie Heim Vögtlin fellowshio (SNSF fellowship)
2008-2009 - Roche foundation fellowship
2005-2008 - FEBS longterm fellowship
2003-2004 - EMBL Fellowship
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Publications
28. Expansion Microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid structure. Bertiaux A., Balestra A., Bournonville L., Brochet M. *, Guichard P. *, Hamel V. * . Submitted
27. The architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography. Klena N.†, Le Guennec M.†, Tassin AM, Van den Hoek H., .Erdmann PS, Schaffer M., Geimer S., Aeschlimann G., Kovacik L., Goldie KN, Stahlberg H. , Engel BD*, Hamel V.* and Guichard P.* Submitted.
25. Ultrastructure Expansion Microscopy (U-ExM)., , Methods in Cell Biology. July 2020.
24. Improving the resolution of fluorescence nanoscopy using post-expansion labeling microscopy. Hamel V.* and Guichard P.* Methods in Cell Biology, In press
23. Molecular resolution imaging by post-labeling expansion single molecular localization microscopy (Ex-SMLM)., , , , , Nature communications. July 2020.
22. Homogeneous multifocal excitation for high-throughput super-resolution imaging.Mahecic D., Gambarotto D., Douglass K.M., Fortun D., Banterle N., Le Guennec M., Ibrahim K., Gonczy P., Hamel V., Guichard P.., Manley S. Nature Methods, June 2020. Link open access
Tosetti N., Dos Santos Pacheco N., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. eLife. May 2020 Link open access
20. A helical inner scaffold provides a structural basis for centriole cohesion. Le Guennec M†, Klena N.†, Gambarotto D.†, Laporte MH †, Tassin AM, Van den Hoek H., Erdmann PS, Schaffer M., Kovacik L., Borgers S., Goldie KN, Stahlberg H., Bornens M., Azimzadeh J., Engel BD#, Hamel V.* and Guichard P.* Science Advances. Feb 2020
19. Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. M. Schmidt-Cernohorska, I. Zhernov, E. Steib, M. Le Guennec1, R. Achek, S. Borgers, D. Demurtas, L. Mouawad, Z. Lansky, V. Hamel* and P. Guichard*. Science. Jan 2019
18. Imaging cellular ultrastructures using expansion microscopy (U-ExM). Gambarotto D. ##, Zwettler F. U. ##, Le Guennec M., Schmidt-Cernohorska M. , Fortun D. , Borgers S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E.S. , Sauer M. *, Hamel V. * and Guichard P.*. Nature Methods. 2019
17. Isolation, Imaging and fluorescent single particle reconstruction of Chlamydomonas centrioles . Klena N.§, Gambarotto D. §, Le Guennec M. , Borgers S., Guichard P.*, Hamel V.* JoVE. (139), e58109, doi:10.3791/58109
16. Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy. Fortun D, Guichard P, Hamel V, Sorzano COS, Banterle N, Gonczy P, Unser M. IEEE Trans Med Imaging. 2018 May;37(5):1235-1246.
15. Rise of the Cartwheel: Seeding the Centriole Organelle. Guichard P, Hamel V, Gönczy P. Bioessays. 2018 Apr;40(4):e1700241.
14. Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, Busso C, Olieric N, Sorzano COS, Steinmetz MO, Guichard P*, Gönczy P.* Curr Biol. 2017 Aug 21;27(16):2486-2498.e6.
13. Cell-free reconstitution reveals centriole cartwheel assembly mechanisms. Guichard P*, Hamel V*, Le Guennec M, Banterle N, Iacovache I, Nemčíková V, Flückiger I, Goldie KN, Stahlberg H, Lévy D, Zuber B, Gönczy P. Nat Commun. 2017 Mar 23;8:14813.
12. Computational support for a scaffolding mechanism of centriole assembly. Klein HC, Guichard P, Hamel V, Gönczy P, Schwarz US. Sci Rep. 2016 Jun 8;6:27075.
11. SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Hilbert M*, Noga A*, Frey D*, Hamel V*, Guichard P, Kraatz SH, Pfreundschuh M, Hosner S, Flückiger I, Jaussi R, Wieser MM, Thieltges KM, Deupi X, Müller DJ, Kammerer RA, Gönczy P, Hirono M, Steinmetz MO. Nat Cell Biol. 2016 Apr;18(4):393-403.
10. Isolation, cryotomography, and three-dimensional reconstruction of centrioles. Guichard P, Hamel V, Neves A, Gönczy P. Methods Cell Biol. 2015;129:191-209.
9. Correlative multicolor 3D SIM and STORM microscopy. Hamel V, Guichard P, Fournier M, Guiet R, Flückiger I, Seitz A, Gönczy P. Biomed Opt Express. 2014 Aug 29;5(10):3326-36.
8. Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells. Keller D, Orpinell M, Olivier N, Wachsmuth M, Mahen R, Wyss R, Hachet V, Ellenberg J, Manley S, Gönczy P. J Cell Biol. 2014 Mar 3;204(5):697-712.
7. Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. Guichard P, Hachet V, Majubu N, Neves A, Demurtas D, Olieric N, Fluckiger I, Yamada A, Kihara K, Nishida Y, Moriya S, Steinmetz MO, Hongoh Y, Gönczy P. Curr Biol. 2013 Sep 9;23(17):1620-8.
6. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Hilbert M*, Erat MC*, Hachet V*, Guichard P, Blank ID, Flückiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gönczy P, Vakonakis I. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8.
5. Cartwheel architecture of Trichonympha basal body. Guichard P, Desfosses A, Maheshwari A, Hachet V, Dietrich C, Brune A, Ishikawa T, Sachse C, Gönczy P. Science. 2012 Aug 3;337(6094):553.
4. The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos. Hachet V, Busso C, Toya M, Sugimoto A, Askjaer P, Gönczy P. Mol Biol Cell. 2012 Aug;23(16):3111-21.
3. Centrosomes promote timely mitotic entry in C. elegans embryos. Hachet V, Canard C, Gönczy P. Dev Cell. 2007 Apr;12(4):531-41.
2. Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3. Faul C, Hüttelmaier S, Oh J, Hachet V, Singer RH, Mundel P. J Cell Biol. 2005 May 9;169(3):415-24.
1. Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro. Hachet V, Köcher T, Wilm M, Mattaj IW. EMBO J. 2004 Apr 7;23(7):1526-35.